2D section images to 3D atlas

Tools for anatomical landmark based registration of mouse and rat 2D brain section images to 3D reference atlases.

Registration tools for 2D histological rodent brain section images to a 3D reference atlas

Efficient semiautomatic affine registration of serial 2D images to a 3D reference atlas.
Optimise initial registration using non-linear adjustments.
Easily customise atlas overlay plates for your serial sections.
Save the 3D spatial locations of all your sections in the atlas space for further spatial analyses.

Registering different experimental neuroscience datasets to the same reference atlas allows you to spatially integrate, analyse and navigate these datasets within a standardised coordinate system. 2D histological section images of rat or mouse brains can be quickly and easily spatially registered to 3D reference atlases using QuickNII; furthermore, this registration can be fine-tuned using non-linear adjustments in VisuAlign. Both tools are downloadable desktop applications with user-friendly graphical interface and tutorials for getting started. Online versions of these tools, WebAlign and WebWarp are now also available on the EBRAINS platform.

Register mouse and rat 2D brain images to a 3D reference atlas

You can use QuickNII and VisuAlign for:

Identification of brain regions in histological brain section image series
Mapping the position of 3D reconstructed neurons
Cell count analysis using the QUINT workflow

Examples of use

Participate in the 2D image registration community

Are you interested in atlas based spatial quantification? Discover our registration tools and interact with us for personalised advice on how to integrate your neuroscience data with atlases from mice and rats.