ZetaStitcher

ZetaStitcher was designed to stitch the large volumetric datasets that are produced, for example, with Light-Sheet Microscopy when imaging large samples (such as a whole mouse brain) at high resolution. This tool computes optimal alignment of adjacent tiles by evaluating the cross-correlation of overlapping areas at selected stack depths. This ensures a high throughput, since a large dataset need not be processed in its entirety. Cross-correlation is computed efficiently by means of FFT. The software is fully written in Python and exposes an Application Programming Interface (API) that can be used to perform queries on the stitched dataset for further processing.

Create an account

EBRAINS is open and free. Sign up now for complete access to our tools and services.